AustralieFrV1, Main, Exploration, bibRecord, 007597

Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests

Identifieur interne : 007597 ( Main/Exploration ); précédent : 007596; suivant : 007598

Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests

Auteurs : Cedric Badiou [France] ; Oana Dumitrescu [France] ; Narelle George [Australie] ; Andrea R. N. Forbes [États-Unis] ; Eleanna Drougka [Grèce] ; KIAN SING CHAN [Singapour] ; Nadjia Ramdani-Bouguessa [Algérie] ; Helene Meugnier [France] ; Michele Bes [France] ; Francois Vandenesch [France] ; Jerome Etienne [France] ; LI YANG HSU [Singapour] ; Mohamed Tazir [Algérie] ; Iris Spiliopoulou [Grèce] ; Graeme R. Nimmo [Australie] ; Kristina G. Hulten [États-Unis] ; Gerard Lina [France]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2010.

RBID : Pascal:10-0219375

Descripteurs français

Pascal (Inist)
- Staphylococcus aureus, Technique rapide, Leucocidine, Isolat clinique, Technique ELISA.

English descriptors

KwdEn :
- Clinical isolate, ELISA assay, Leucocidine, Rapid technique, Staphylococcus aureus.

Abstract

Staphylococcus aureus strains producing Panton-Valentine leukocidin (PVL) have been epidemiologically linked to specific human infections. To evaluate immunological tests that may be used to diagnose infections with PVL-producing strains, we prospectively collected pus, respiratory tract specimens, and joint fluid specimens from which S. aureus had been isolated in clinical laboratories in six countries. An enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic test (ICT) targeting LukS-PV were performed directly with clinical samples for the detection of PVL. The same tests were applied to S. aureus culture supernatants. The corresponding S. aureus isolates were characterized by PCR for the presence of the PVL locus (lukS-PV and lukF-PV) and the mecA gene. A total of 185 samples from 144 skin infections, 23 bone and joint infections, and 18 lower respiratory tract infections were analyzed. By PCR, 72/185 S. aureus isolates were PVL locus positive (PVL⁺); 28 of these were also mecA positive. PVL was detected in the supernatants of all PVL⁺ strains by both ELISA and an ICT, while no signal was observed with PVL-negative strains. The PVL concentrations in human clinical samples that grew PVL⁺ strains ranged from 0 to 399 μg/ml by ELISA. By the use of 0.015 μg/ml of PVL as a cutoff value, PVL was detected in 65/72 (90%) of the clinical samples by ELISA. The sensitivity and specificity of the ELISA test were 90% and 100%, respectively. By the ICT, PVL was detected in 57/72 (79%) of the samples, and the sensitivity and specificity of ICT were 79% and 100%, respectively. PVL is expressed by S. aureus during human infection, and a PVL-specific ELISA and ICT could be reliable tests for the diagnosis of infections caused by PVL-producing strains.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2849559

Affiliations:

Le document en format XML

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<author><name sortKey="Forbes, Andrea R N" sort="Forbes, Andrea R N" uniqKey="Forbes A" first="Andrea R. N." last="Forbes">Andrea R. N. Forbes</name>
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<author><name sortKey="Drougka, Eleanna" sort="Drougka, Eleanna" uniqKey="Drougka E" first="Eleanna" last="Drougka">Eleanna Drougka</name>
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<author><name sortKey="Ramdani Bouguessa, Nadjia" sort="Ramdani Bouguessa, Nadjia" uniqKey="Ramdani Bouguessa N" first="Nadjia" last="Ramdani-Bouguessa">Nadjia Ramdani-Bouguessa</name>
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<author><name sortKey="Meugnier, Helene" sort="Meugnier, Helene" uniqKey="Meugnier H" first="Helene" last="Meugnier">Helene Meugnier</name>
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<author><name sortKey="Vandenesch, Francois" sort="Vandenesch, Francois" uniqKey="Vandenesch F" first="Francois" last="Vandenesch">Francois Vandenesch</name>
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<author><name sortKey="Etienne, Jerome" sort="Etienne, Jerome" uniqKey="Etienne J" first="Jerome" last="Etienne">Jerome Etienne</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
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<s3>FRA</s3>
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<settlement type="city">Lyon</settlement>
</placeName>
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<author><name sortKey="Li Yang Hsu" sort="Li Yang Hsu" uniqKey="Li Yang Hsu" last="Li Yang Hsu">LI YANG HSU</name>
<affiliation wicri:level="4"><inist:fA14 i1="09"><s1>Department of Medicine, National University of Singapore</s1>
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<s3>SGP</s3>
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<author><name sortKey="Tazir, Mohamed" sort="Tazir, Mohamed" uniqKey="Tazir M" first="Mohamed" last="Tazir">Mohamed Tazir</name>
<affiliation wicri:level="3"><inist:fA14 i1="08"><s1>Service de Microbiologie, Centre Hospitalo-Universitaire Mustapha Bacha</s1>
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<author><name sortKey="Spiliopoulou, Iris" sort="Spiliopoulou, Iris" uniqKey="Spiliopoulou I" first="Iris" last="Spiliopoulou">Iris Spiliopoulou</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Microbiology, School of Medicine, University of Patras</s1>
<s2>Patras</s2>
<s3>GRC</s3>
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<sZ>14 aut.</sZ>
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<country>Grèce</country>
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<author><name sortKey="Nimmo, Graeme R" sort="Nimmo, Graeme R" uniqKey="Nimmo G" first="Graeme R." last="Nimmo">Graeme R. Nimmo</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Division of Microbiology, Pathology Queensland Central Laboratory, Herston Hospitals Complex, Herston</s1>
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<s3>AUS</s3>
<sZ>3 aut.</sZ>
<sZ>15 aut.</sZ>
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<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hulten, Kristina G" sort="Hulten, Kristina G" uniqKey="Hulten K" first="Kristina G." last="Hulten">Kristina G. Hulten</name>
<affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Department of Pediatrics, Baylor College of Medicine</s1>
<s2>Houston, Texas</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
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</author>
<author><name sortKey="Lina, Gerard" sort="Lina, Gerard" uniqKey="Lina G" first="Gerard" last="Lina">Gerard Lina</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
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<country>France</country>
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<settlement type="city">Lyon</settlement>
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<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
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<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
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<idno type="wicri:Area/Main/Exploration">007597</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests</title>
<author><name sortKey="Badiou, Cedric" sort="Badiou, Cedric" uniqKey="Badiou C" first="Cedric" last="Badiou">Cedric Badiou</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dumitrescu, Oana" sort="Dumitrescu, Oana" uniqKey="Dumitrescu O" first="Oana" last="Dumitrescu">Oana Dumitrescu</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="George, Narelle" sort="George, Narelle" uniqKey="George N" first="Narelle" last="George">Narelle George</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Division of Microbiology, Pathology Queensland Central Laboratory, Herston Hospitals Complex, Herston</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>3 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Forbes, Andrea R N" sort="Forbes, Andrea R N" uniqKey="Forbes A" first="Andrea R. N." last="Forbes">Andrea R. N. Forbes</name>
<affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Department of Pediatrics, Baylor College of Medicine</s1>
<s2>Houston, Texas</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Drougka, Eleanna" sort="Drougka, Eleanna" uniqKey="Drougka E" first="Eleanna" last="Drougka">Eleanna Drougka</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Microbiology, School of Medicine, University of Patras</s1>
<s2>Patras</s2>
<s3>GRC</s3>
<sZ>5 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
<country>Grèce</country>
<wicri:noRegion>Patras</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kian Sing Chan" sort="Kian Sing Chan" uniqKey="Kian Sing Chan" last="Kian Sing Chan">KIAN SING CHAN</name>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Pathology, Singapore General Hospital</s1>
<s2>Singapore</s2>
<s3>SGP</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ramdani Bouguessa, Nadjia" sort="Ramdani Bouguessa, Nadjia" uniqKey="Ramdani Bouguessa N" first="Nadjia" last="Ramdani-Bouguessa">Nadjia Ramdani-Bouguessa</name>
<affiliation wicri:level="3"><inist:fA14 i1="08"><s1>Service de Microbiologie, Centre Hospitalo-Universitaire Mustapha Bacha</s1>
<s2>Alger</s2>
<s3>DZA</s3>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
<country>Algérie</country>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Meugnier, Helene" sort="Meugnier, Helene" uniqKey="Meugnier H" first="Helene" last="Meugnier">Helene Meugnier</name>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bes, Michele" sort="Bes, Michele" uniqKey="Bes M" first="Michele" last="Bes">Michele Bes</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Vandenesch, Francois" sort="Vandenesch, Francois" uniqKey="Vandenesch F" first="Francois" last="Vandenesch">Francois Vandenesch</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Etienne, Jerome" sort="Etienne, Jerome" uniqKey="Etienne J" first="Jerome" last="Etienne">Jerome Etienne</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li Yang Hsu" sort="Li Yang Hsu" uniqKey="Li Yang Hsu" last="Li Yang Hsu">LI YANG HSU</name>
<affiliation wicri:level="4"><inist:fA14 i1="09"><s1>Department of Medicine, National University of Singapore</s1>
<s2>Singapore</s2>
<s3>SGP</s3>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
</author>
<author><name sortKey="Tazir, Mohamed" sort="Tazir, Mohamed" uniqKey="Tazir M" first="Mohamed" last="Tazir">Mohamed Tazir</name>
<affiliation wicri:level="3"><inist:fA14 i1="08"><s1>Service de Microbiologie, Centre Hospitalo-Universitaire Mustapha Bacha</s1>
<s2>Alger</s2>
<s3>DZA</s3>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
<country>Algérie</country>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Spiliopoulou, Iris" sort="Spiliopoulou, Iris" uniqKey="Spiliopoulou I" first="Iris" last="Spiliopoulou">Iris Spiliopoulou</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Microbiology, School of Medicine, University of Patras</s1>
<s2>Patras</s2>
<s3>GRC</s3>
<sZ>5 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
<country>Grèce</country>
<wicri:noRegion>Patras</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nimmo, Graeme R" sort="Nimmo, Graeme R" uniqKey="Nimmo G" first="Graeme R." last="Nimmo">Graeme R. Nimmo</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Division of Microbiology, Pathology Queensland Central Laboratory, Herston Hospitals Complex, Herston</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>3 aut.</sZ>
<sZ>15 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hulten, Kristina G" sort="Hulten, Kristina G" uniqKey="Hulten K" first="Kristina G." last="Hulten">Kristina G. Hulten</name>
<affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Department of Pediatrics, Baylor College of Medicine</s1>
<s2>Houston, Texas</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lina, Gerard" sort="Lina, Gerard" uniqKey="Lina G" first="Gerard" last="Lina">Gerard Lina</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Université Lyon 1, Centre National de Référence des Staphylocoques</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>INSERM U851, IFR128</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Hospices Civils de Lyon</s1>
<s2>Lyon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>17 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clinical isolate</term>
<term>ELISA assay</term>
<term>Leucocidine</term>
<term>Rapid technique</term>
<term>Staphylococcus aureus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Staphylococcus aureus</term>
<term>Technique rapide</term>
<term>Leucocidine</term>
<term>Isolat clinique</term>
<term>Technique ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Staphylococcus aureus strains producing Panton-Valentine leukocidin (PVL) have been epidemiologically linked to specific human infections. To evaluate immunological tests that may be used to diagnose infections with PVL-producing strains, we prospectively collected pus, respiratory tract specimens, and joint fluid specimens from which S. aureus had been isolated in clinical laboratories in six countries. An enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic test (ICT) targeting LukS-PV were performed directly with clinical samples for the detection of PVL. The same tests were applied to S. aureus culture supernatants. The corresponding S. aureus isolates were characterized by PCR for the presence of the PVL locus (lukS-PV and lukF-PV) and the mecA gene. A total of 185 samples from 144 skin infections, 23 bone and joint infections, and 18 lower respiratory tract infections were analyzed. By PCR, 72/185 S. aureus isolates were PVL locus positive (PVL<sup>+</sup>
); 28 of these were also mecA positive. PVL was detected in the supernatants of all PVL<sup>+</sup>
 strains by both ELISA and an ICT, while no signal was observed with PVL-negative strains. The PVL concentrations in human clinical samples that grew PVL<sup>+</sup>
 strains ranged from 0 to 399 μg/ml by ELISA. By the use of 0.015 μg/ml of PVL as a cutoff value, PVL was detected in 65/72 (90%) of the clinical samples by ELISA. The sensitivity and specificity of the ELISA test were 90% and 100%, respectively. By the ICT, PVL was detected in 57/72 (79%) of the samples, and the sensitivity and specificity of ICT were 79% and 100%, respectively. PVL is expressed by S. aureus during human infection, and a PVL-specific ELISA and ICT could be reliable tests for the diagnosis of infections caused by PVL-producing strains.</div>
</front>
</TEI>
<affiliations><list><country><li>Algérie</li>
<li>Australie</li>
<li>France</li>
<li>Grèce</li>
<li>Singapour</li>
<li>États-Unis</li>
</country>
<region><li>Auvergne-Rhône-Alpes</li>
<li>Rhône-Alpes</li>
<li>Texas</li>
<li>Wilaya d'Alger</li>
</region>
<settlement><li>Alger</li>
<li>Lyon</li>
</settlement>
<orgName><li>Université nationale de Singapour</li>
</orgName>
</list>
<tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Badiou, Cedric" sort="Badiou, Cedric" uniqKey="Badiou C" first="Cedric" last="Badiou">Cedric Badiou</name>
</region>
<name sortKey="Badiou, Cedric" sort="Badiou, Cedric" uniqKey="Badiou C" first="Cedric" last="Badiou">Cedric Badiou</name>
<name sortKey="Bes, Michele" sort="Bes, Michele" uniqKey="Bes M" first="Michele" last="Bes">Michele Bes</name>
<name sortKey="Bes, Michele" sort="Bes, Michele" uniqKey="Bes M" first="Michele" last="Bes">Michele Bes</name>
<name sortKey="Bes, Michele" sort="Bes, Michele" uniqKey="Bes M" first="Michele" last="Bes">Michele Bes</name>
<name sortKey="Dumitrescu, Oana" sort="Dumitrescu, Oana" uniqKey="Dumitrescu O" first="Oana" last="Dumitrescu">Oana Dumitrescu</name>
<name sortKey="Dumitrescu, Oana" sort="Dumitrescu, Oana" uniqKey="Dumitrescu O" first="Oana" last="Dumitrescu">Oana Dumitrescu</name>
<name sortKey="Dumitrescu, Oana" sort="Dumitrescu, Oana" uniqKey="Dumitrescu O" first="Oana" last="Dumitrescu">Oana Dumitrescu</name>
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   |texte=   Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests
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This area was generated with Dilib version V0.6.33.
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Serveur d'exploration sur les relations entre la France et l'Australie

Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests

Rapid Detection of Staphylococcus aureus Panton-Valentine Leukocidin in Clinical Specimens by Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests

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	Serveur d'exploration sur les relations entre la France et l'Australie
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